Table 2.
Primers used in this study.
| Primers | Oligonucleotide sequences (5′-3′)∗ |
|---|---|
| pHcp-up-SacI | CCCGAGCTCAGTCCGTCGCCATCAAATAG |
| pHcp-dn-BamHI | CGGGATCCGAGTTTGACCTTCGATAGAG |
| pHcp-A-up-SacI | CCCGAGCTCTGAGAATAGCCTTCCTTGAC |
| pHcp-B-up-SacI | CCCGAGCTCGTGCCACCTTTGGCTACGTT |
| pHcp-B-dn-SpeI | GGACTAGTGAGTTTGACCTTCGATAGAG |
| pHcp-C′-dn | CCAACTGGGCAATAACAAATAATCAATAAGTT AGCGC |
| pHcp-C′-up | TTGATTATTTGTTATTGCCCAGTTGGCAAGTTAT |
| pHcp-A-M-up | GGCAAGGTTTTAAATATCACTAATACCTTTT AAAAGAATGCCAAAGTGG |
| pHcp-A-M-dn | TTTGGCATTCTTTTAAAAGGTATTAGTGATA TTTAAAACCTTGCCATTAGA |
| pT6SS2-up-SacI | ACGAGCTCACCATGATCTGTTCTGGGAT |
| pT6SS2-dn-BamHI | CGGGATCCTTAGGAGCTACACTTCCTTC |
| pT6SS2-1M-dn | TCTATTCATTTAATCATGTTTACGTGCACAAA AATCACAAGAATA |
| pT6SS2-1M-up | TGATTTTTGTGCACGTAAACATGATTAAATGAA TAGAATGTGCTCG |
| pT6SS2-2M-dn | TTTTTAGTAAATATCACTTCTACCAATTGATTA ATTCACCCGACTT |
| pT6SS2-2M-up | AATTAATCAATTGGTAGAAGTGATATTTACTAA AAATCAAATAAGATA |
| pT6SS2-3M-dn | AATAGTTGTGTCAATATCACCTTGATAATACAC ATTAGAAATATC |
| pT6SS2-3M-up | TGTGTATTATCAAGGTGATATTGACACAACTAT TTCATTGACAAC |
| vflihfA-F1-up-BamHI | CGGGATCCGGAGAGTGAATGAGCCTA |
| vflihfA-F1-dn | TTTTATGGCGGTCGTAAAAAGACCGAGC |
| vflihfA-F2-up | TTTTTACGACCGCCATAAAACTTCCCTC |
| vflihfA-F2-dn-XhoI | CCGCTCGAGTCACCCTGAGCTTGAACG |
| vflihfB-F1-up-BamHI | CGGGATCCTGGTTCGTCGACAAGCTG |
| vflihfB-F1-dn | AAACTATGACCGAAAACATTTGATTTACG |
| vflihfB-F2-up | AATGTTTTCGGTCATAGTTTCCCTCATCG |
| vflihfB-F2-dn-XhoI | CCGCTCGAGCAGTCATTCGCTGAAGCAC |
| vflihfA-F-XhoI | CCGCTCGAGTTACGACTTTTTAATGTTC |
| vflihfA-R-NdeI | GGAATTCCATATGGCGCTCACAAAGGC |
| vflihfB-F-XhoI | CCGCTCGAGTCAAATGTTTTCGTTTACA |
| vflihfB-R-NdeI | GGAATTCCATATGACTAAGTCTGAATTG |
| VF-recA-qPCR-up | ACCGAGTCAACGACGATAAC |
| VF-recA-qPCR-dn | TGATGAACTGCTGGTGTCTC |
| qvipA/tssB2-F | CTGACGACAACAGTGAAGAAC |
| qvipA/tssB2-R | TGCGAAGCCACAGAATCC |
| hcp-qPCR-F-com | TCGGCGATTCATTCGTT |
| hcp-qPCR-R-com | CAGTTCAACCGTCGTCATCT |
| vgrG-qPCR-F-AB | GCATCTTCCAACTCAACAC |
| vgrG-qPCR-R-AB | GTACACCAGCCCTTCTTC |
| VF-vasK-qPCR-F | ACATCCAACGCCAATACG |
| VF-vasK-qPCR-R | CAATCGCAGTGAAGACAAC |
| VF-vasH-qPCR-F | GGTAATCGGATACTGGAAC |
| VF-vasH-qPCR-R | CATGTCAACTTGCTGGAT |
| HcpA-up-Biotin | TGAGAATAGCCTTCCTTGAC |
| HcpA-dn-Biotin | GAGTTTGACCTTCGATAGAG |
| T6SS2-up-Biotin | ACCATGATCTGTTCTGGGAT |
| T6SS2-dn-Biotin | TTAGGAGCTACACTTCCTTC |
∗The underlined bases indicate the restriction enzyme sites.