Table 1.

The immunization procedure for BALB/c mice.

Immunization times	Immunogen	Immunologic adjuvant	Injection route	Dose (μg)
First 7 weeks of age	DTMUV-E protein	Freund’s complete adjuvant	Multiple injection sites in the abdomen hypodermic	100
Second 8 weeks of age	DTMUV-E protein	Freund’s incomplete adjuvant	Multiple injection sites in the abdomen hypodermic	100
Third 9 weeks of age	DTMUV-E protein	Freund’s incomplete adjuvant	Multiple injection sites in the abdomen hypodermic	100
Fourth/strengthen immunity 10 weeks of age	DTMUV-E protein	None	Intraperitoneal injection	80