No significant effect of obvious pyramidal cell loss on seizure frequency in epileptic pilocarpine-treated mice. Nissl staining of the hippocampus in a control mouse (a1), an epileptic mouse with obvious hilar neuron loss (a2), and an epileptic mouse with obvious loss of neurons in the hilus, CA1, and CA3 (a3). h = hilus. (b) Percentage of epileptic pilocarpine-treated mice with and without obvious pyramidal cell loss. (c) No significant difference in seizure frequency of mice with and without obvious pyramidal cell loss (p = 0.426, Mann–Whitney rank sum test)