At the end of 21 days of treatment with Mn, VPA, NaB as previously mentioned, tissues from cerebral cortex and cerebellum were prepared and measured for GLT-1 mRNA levels by qPCR. (A, B) GLT-1 mRNA levels in cerebral cortex (4A) and cerebellum (4B) from mouse brain treated with Mn, VPA or Mn plus VPA. (C, D) GLT-1 mRNA levels in cerebral cortex (4C) and cerebellum (4D) from mouse brain treated with Mn, NaB, or Mn plus NaB. ##, p<0.01; ###, p<0.001; **, p<0.01; ***, p<0.001 (ANOVA followed by Tukey’s post hoc test; n = 3).