Table 1.

Body weight changes in mice after treated with Mn, VPA, NaB, Mn plus VPA, or Mn plus NaB for 21 days

Treatment Groups	Day 1 Average Weight (g, n=8)	Day 21 Average Weight (g, n=8)	Weight Gain (+)/Loss (−) (g)
Control	23.04 ± 1.77	26.58 ± 1.74	+3.55 ± 0.94
Mn	19.54 ± 3.47	18.11 ± 2.06	−1.43 ± 2.02
VPA	22.58 ± 1.27	24.60 ± 1.82	+2.03 ± 1.16
VPA + Mn	21.99 ± 0.85	22.02 ± 0.99	+0.03 ± 0.43
NaB	22.48 ± 1.19	23.45 ± 1.65	+0.97 ± 1.37
NaB + Mn	21.90 ± 1.73	20.07 ± 1.41	−1.83 ± 0.44

Mice were pretreated with VPA (100 μl/200 mg/kg, i.p. daily) or with NaB (100 μl/1200 mg/kg, i.p. daily) 30 min prior to Mn exposure (2 μl/30mg/kg, intranasal instillation, daily) for consecutive 21 days. Body weights were measured throughout the experiment and weight gain recorded on day 1 and day 21. Results represent mean ± S.E.M derived from eight independent experiments and each of their weights are expressed in grams (g). Statistical analysis was performed by two-way ANOVA repeated measures, followed by Tukey’s post-hoc test, (n=8).