Table 3.
Bone marrow cell immunophenotyping in female B6C3F1/N mice exposed to STD in the drinking water for 28 days
| Treatment | Cells/Femur | B220+ | CD3+ | Gr-1+ (Int) | Gr-1+ (High) | Gr-1+ (Total) | CD11b+ | TER-119+ |
|---|---|---|---|---|---|---|---|---|
| Absolute Values (x106) | ||||||||
| Vehicle | 7.47 ± 0.61 | 1.5 ± 0.1 | 0.1 ± 0.1 | 0.9 ± 0.1 | 2.6 ± 0.2 | 3.5 ± 0.3 | 3.3 ± 0.2 | 2.2 ± 0.2 |
| STD | ||||||||
| 125 mg/L | 8.82 ± 0.52 | 1.7 ± 0.2 | 0.1 ± 0.1 | 1.0 ± 0.1 | 3.2 ± 0.2 | 4.1 ± 0.2 | 3.8 ± 0.2 | 2.5 ± 0.2 |
| 250 mg/L | 9.44 ± 0.68 | 2.0 ± 0.2 | 0.1 ± 0.1 | 1.1 ± 0.1 | 3.2 ± 0.2 | 4.2 ± 0.2 | 3.9 ± 0.2 | 2.8 ± 0.2 |
| 500 mg/L | 8.60 ± 0.64 | 1.8 ± 0.1 | 0.1 ± 0.1 | 1.0 ± 0.1 | 2.7 ± 0.3 | 3.7 ± 0.3 | 3.5 ± 0.3 | 2.7 ± 0.2 |
| 1000 mg/L | 7.25 ± 0.64 | 1.7 ± 0.2 | 0.1 ± 0.1 | 0.8 ± 0.1 | 2.4 ± 0.2 | 3.2 ± 0.2 | 3.1 ± 0.3 | 2.1 ± 0.2 |
| 2000 mg/L | 10.57 ± 1.01 | 2.3 ± 0.3 | 0.2 ± 0.1* | 1.1 ± 0.1 | 3.5 ± 0.3 | 4.6 ± 0.4 | 4.5 ± 0.4 | 3.3 ± 0.3 |
| CPS (50 mg/kg) | 4.07 ± 0.44** | 0.1 ± 0.1** | 0.1 ± 0.1** | 0.1 ± 0.1** | 0.1 ± 0.1** | 0.2 ± 0.1** | 0.2 ± 0.1** | 3.6 ± 0.4** |
| Trend Analysis | NS | p ≤ 0.05 | p ≤ 0.05 | NS | NS | NS | NS | NS |
| Percent Values | ||||||||
| Vehicle | 20.1 ± 0.8 | 1.3 ± 0.1 | 11.4 ± 0.3 | 34.8 ± 1.0 | 46.1 ± 1.2 | 43.9 ± 1.0 | 29.3 ± 0.8 | |
| STD | ||||||||
| 125 mg/L | 18.9 ± 1.8 | 1.5 ± 0.2 | 11.4 ± 0.3 | 35.9 ± 1.3 | 47.2 ± 1.5 | 43.4 ± 1.6 | 28.6 ± 1.9 | |
| 250 mg/L | 20.6 ± 1.1 | 1.4 ± 0.1 | 11.3 ± 0.4 | 34.3 ± 1.3 | 45.5 ± 1.5 | 41.2 ± 1.2 | 29.5 ± 1.0 | |
| 500 mg/L | 21.5 ± 1.0 | 1.5 ± 0.1 | 11.5 ± 0.2 | 31.2 ± 1.0 | 42.6 ± 1.0 | 40.5 ± 0.9 | 31.7 ± 0.6 | |
| 1000 mg/L | 23.7 ± 0.9 | 1.5 ± 0.1 | 11.4 ± 0.2 | 32.9 ± 1.3 | 44.2 ± 1.4 | 42.5 ± 0.9 | 29.1 ± 1.0 | |
| 2000 mg/L | 21.2 ± 1.2 | 1.5 ± 0.1 | 10.7 ± 0.3 | 32.9 ± 1.2 | 43.6 ± 1.3 | 42.7 ± 0.8 | 31.7 ± 1.1 | |
| CPS (50 mg/kg) | 1.4 ± 0.1** | 1.3 ± 0.1 | 2.6 ± 0.2** | 2.7 ± 0.3** | 5.3 ± 0.4** | 4.5 ± 0.4** | 88.1 ± 0.5** | |
| Trend Analysis | p ≤ 0.05 | NS | NS | p ≤ 0.05 | p ≤ 0.05 | NS | NS | |
B220+ = B-lymphocyte lineage, CD3+ = T-lymphocyte lineage, Gr-1+ (Int) = Neutrophils, myelocytes and other immature progenitors, Gr-1+ (High) = Neutrophils, Gr-1+ (Total) = Total Gr-1+ cells, CD11b+ = Granulocytes and monocytes, TER-119+ = Erythroid lineage, STD = Sodium Tungstate Dihydrate, CPS = Cyclophosphamide (positive control)
Values represent the mean (± SE) from 8 animals/group;
*
p ≤ 0.05;
**
p ≤ 0.01
NS = Not significant