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. 2018 Apr 1;9(2):235–248. doi: 10.14336/AD.2017.0328

Figure 6.

Figure 6.

TOPK influences inflammatory response in BV2 cells following LPS plus IFN-γ stimulation. (A) ELISA analysis of TNF-α, IL-6 and IL-1β levels in the culture medium of LPS (100 ng/ml) plus IFN-γ (20 ng/ml)-induced BV2 cells for 24 h. (B) ELISA analysis of TGF-β level in the culture medium of LPS (100 ng/ml) plus IFN-γ (20 ng/ml)-induced BV2 cells for 24 h. (C) Determination of mRNA expression of M2 phenotype, TGF-β and SOCS3 by RT-PCR. LPS, lipopolysaccharide. Data are expressed as the mean ± SEM. N=6. *p < 0.05 versus control. #p < 0.05 versus LPS. Statistics: Tukey’s honest significance test.