Figure 8.

Combination of ACY-1215 and pasireotide increases ciliary length and frequency of their occurrences in cholangiocytes of polycystic kidney (PCK) rats. A: Representative images of liver sections from untreated and drug-treated PCK rats are stained with antibody to ciliary marker, acetylated α-tubulin (red). Nuclei are stained in blue with DAPI. Area depicted by asterisk is shown with a higher power underneath. B and C: The length of primary cilia (B) and the percentage of ciliated cholangiocytes (C) increase in response to treatment with ACY-1215, pasireotide, and drug combination. Randomly selected microscopic fields from each group of untreated and drug-treated PCK rats were analyzed. Data are expressed as means ± SD. n = 5 microscopic fields. ^∗∗P < 0.01, ^∗∗∗∗P < 0.0001. Scale bars = 10 μm (A, main images and insets).