Figure 5.

Metamorphosis of the SEZ. All panels show Z-projections of frontal confocal sections of pupal brain at 12hrs APF (P12; left column; a, d, g, j, m), 24hrs (P24; middle column; b, e, h, k, n) and adult brain (Ad; right column; c, f, i, l, o). Preparations are labeled with anti-Neuroglian (green on left halves of panels; labels secondary axon tracts), and anti-DN-cadherin (neuropil; magenta on left halves of panels; white on right halves). Z-projections represent different levels along the antero-posterior axis [(a–c) tritocerebrum (TR); (d–e) mandibula (MD); (g–i) maxilla (MX); (j–l) anterior labium (LBant); (m–o) posterior labium (LBpost)]. Hatched lines indicate boundaries between columnar neuropil domains, as described in the text. White arrows point at DN-cadherin-rich core of central neuropil column, targeted by lineages 3 and 7 of all neuromeres. White arrowheads point out DN-cadherin-poor poor periphery of central neuropil column, which corresponds to bundles of long axons (CITd and CITv tracts). Blue arrowhead in (g, h) shows chiasmatic midline crossing of lineage 7_MX. Large yellow arrow in (h, i) indicates location where DN-cadherin-poor CITd and CITv converge at the later neuropil surface. Small yellow arrows point at DN-cadherin-poor CITv bundle. For abbreviations see List of Abbreviations. Bar: 25μm