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. Author manuscript; available in PMC: 2019 Jun 15.
Published in final edited form as: Neuroscience. 2018 Apr 12;381:79–90. doi: 10.1016/j.neuroscience.2018.04.002

Figure 1. Increased Ca2+ currents in Nf1 edited sensory neurons are normalized by t-CNRP1.

Figure 1

(A) Representative micrograph showing neurofibromin levels in DRG neurons transfected with either pSpCas9(BB)-2A-GFP (control gRNA) or pSpCas9(BB)-2A-GFP-Nf1 gRNA plasmids. GFP fluorescence identifies transfected neurons. In this experiment, neuron without GFP has robust expression of neurofibromin, whereas the adjacent neuron with GFP fluorescence (circled) demonstrates significantly decreased neurofibromin expression (marked by an arrow). Scale bar is 10 μm. (B) Representative family of current traces are illustrated for neurons transfected with control or Nf1 gRNA and treated with 10uM t-CNRP1 or DMSO 0.01%. (C) Peak CaV2.2 current density, at +10 mV, in DRG neurons unedited or transfected by Nf1 gRNA containing plasmid and treated with 10 μM t-CNRP1 or DMSO 0.01%. Line shows peak CaV.2.2 current level in Nf1 unedited DRG neurons. Mean ± s.e.m., asterisks denote statistical significance compared with 0.01% DMSO treated cells (p<0.05, non-parametric one-way ANOVA). Recordings are from small and medium DRG neurons.