Table 3. Overall distribution of the KRAS, NRAS, BRAF and TP53 gene mutations detected by next generation sequencing in the 87 colorectal cancer patients analyzed.
| Gene status | N. of cases (%) | Exon location | Mutation (N. of cases) |
|---|---|---|---|
| KRAS | |||
| Wild type | 63 (72%) | ||
| Mutated | 24 (28%) | ||
| Exon 2 | G12D(7), G12V(5), G12A(4), G12S(1), G13D(2) | ||
| Exon 3 | Q61H(1), Q61L(1) | ||
| Exon 4 | A146P(1), A146T(1), K147E(1) | ||
| NRAS | |||
| Wild type | 86 (99%) | ||
| Mutated | 1 (1%) | ||
| Exon 3 | Q61K(1) | ||
| BRAF | |||
| Wild type | 81 (93%) | ||
| Mutated | 6 (7%) | ||
| Exón 15 | V600E (6) | ||
| TP53 | |||
| Wild type | 58 (66%) | ||
| Mutated | 29 (33%) | ||
| Exon 4 | L111R (1), G112del (1) | ||
| Exon 5 | C135F (1), P152AfsTer14 (1), R156H (1), R158C (1), V173L (1), R175H (3), H178PfsTer47 (1), c.376-2A>G splice (1) | ||
| Exon 6 | Q192* (1), R196* (1), L201GfsTer47 (1), S215R (1). | ||
| Exon 7 | R248W (2), R248Q (1), C275Y (1), N247I (1), T253PfsTer92 (1) | ||
| Exon 8 | R282W (3), R273C (1), C277VfsTer68 (1), V272M (1) | ||
| Exon 10 | S366A (1) |
Results expressed as number of cases (percentage).