Table 5. The primer sequences for RT-qPCR.
| Gene | Upstream primer (5′∼3′) | Downstream primer (5′∼3′) |
|---|---|---|
| miR-146a (human) | TATTGGGCAAACAATCAGCA | GCCTGAGACTCTGCCTTCTG |
| miR-146a (rat) | CGCTACTCGTACCGTGAGTAA | GTGCAGGGTCCGAGGT |
| TLR4 (human) | ACTTGGACCTTTCCAGCAAC | TTTAAATGCACCTGGTTGGA |
| TLR4 (rat) | GGACTCTGCCCTGCCACCATTTA | CTTGTGCCCTGTGAGGTCGTTGA |
| NF-κB (human) | TCAAGATCTGCCGACTGAAC | CCTCTTTCTGCACCTTGTCA |
| NF-κB (rat) | GTGCAGAAAGAAGACATTGAGGTG | AGGCTAGGGTCAGCGTATGG |
| IL-1β (human) | GGATATGGAGCAACAAGTGG | ATGTACCAGTTGGGGAACTG |
| IL-6 (human) | ATGAGCTCCTTCTCCACAAGCGC | GAAGAGCCCTCAGGCTGGACTG |
| IL-8 (human) | CATACTCCAAACCTTTCCACCCC | TCAGCCCTCTTCAAAAACTTCTCCA |
| IL-17 (human) | GGGCCTGGCTTCTGTCTGA | AAGTTCGTTCTGCCCCATCA |
| MMP-3 (human) | GGGTGAGGACACCAGCATGA | CAGAGTCGGAGTCCAGCTTC |
| iNOS (human) | TCCAGGAGGACATGCAGCAC | CGCCCTTCCGCAGTTCT |
| COX-2 (human) | ATCTACCCTCCTCAAGTCCC | TACCAGAAGGGCAGGATACAG |
| β-actin (human) | TGGCACCCAGCACAATGAA | CTAAGTCATAGTCCGCCTAGAAGCA |
| β-actin (rat) | GAGAGGGAAATCGTGCGTGAC | CATCTGCTGGAAGGTGGACA |
Notes: RT-qPCR: reverse transcription quantitative polymerase chain reaction; miR-146a: microRNA-146a TLR4: toll-like receptor-4; NF-κB: nuclear factor kappa-light-chain-enhancer of activated B cells; IL: interleukin; MMP: matrix metalloproteinase-3; iNOS: inducible nitric oxide synthase; COX-2: cyclooxygenase-2.