Fig. 7. The effects of compound 2 on mRNA expression of pro-neural bHLH transcriptional activators (Mash1, Ngn2 and NeuroD2) in MEB5 cells after incubation with 2. The mRNA levels were analysed by real-time RT-PCR (normalized to the level of GAPDH). Error bars represent the standard deviation (N = 3). Assays were performed in triplicate. (a) Mash1 (after 24 h), (b) Ngn2 (after 24 h) and (c) NeuroD2 (after 48 h). (d) Possible mechanism of NSC differentiation promoting activity of compound 2.
