Figure 4.
dCas9 gene silencing in Bartonella apis. (A) Schematic assembly of dCas9 plasmids for gene suppression. (B) Fluorescence from chromosomally integrated GFP in PEB0150 in the presence and absence of dCas9 and sgRNA targeting GFP. Background fluorescence of wild-type PEB0150 was subtracted. GFP fluorescence decreased in presence of dCas9 targeting GFP (p = 0.004, Kruskal-Wallis rank sum test). Error bars are 95% confidence intervals (n = 4).