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. Author manuscript; available in PMC: 2018 May 22.
Published in final edited form as: J Cell Biochem. 2017 Jul 31;118(11):3627–3634. doi: 10.1002/jcb.26220

Fig. 1.

Fig. 1

(A) Cell viability of human mesenchymal stem cells possessing a stably integrated inducible caspase 9 (iCasp9) when treated with a chemical inducer of dimerization (CID) or vehicle. All data are reported as the mean ± standard deviation for n = 3. (B) Quantification of alkaline phosphatase (ALP) in W20-17 cells. Media collected from the AdBMP2-transduced hMSCs-iCasp9 cells cultured in the presence of CID or vehicle was added to culture media of W20-17 cells, and 72 h later alkaline phosphatase activity was measured by a chemiluminescent assay. Alkaline phosphatase activity was reported in relative luminescence units (RLUs). All data are reported as the mean ± standard error of the mean for n = 3. * Represents significant difference between groups (P <0.05). (C) LIVE/DEAD staining cultured in complete medium after 24 h. Cells in culture medium (A–C), in the presence of 50 μg of a chemical inducer of dimerization (CID) and 100 ng/ml polyethylenimine (PEI). Maximum intensity projection of green (FITC) channel (A,D,G), red (rhodamine) channel (B,E,H), and merge of all channels (C,F,I). Dead cells appear red and live cells appear green; 20× mag.