Figure 3. SNIRKO mice do not display sensorimotor deficits characteristic of PDN in murine models.

It was hypothesized that the reduction in sensory neuron insulin signaling despite euglycemia would produce a phenotype similar to PDN in mouse models. Similar patterns were observed across males and females between groups and data presented here is combined from males and females. No significant difference between IR^lox/lox and SNIRKO was observed in mechanical sensitivity (A). For thermal sensitivity, there was no significant difference of group between IR^lox/lox and SNIRKO mice upon analysis with 2-way repeated measures ANOVA (B). However, Bonferroni’s post-hoc does show a significant difference between IR^lox/lox and SNIRKO mice at 13 weeks of age. Of note, the thermal threshold for SNIRKO mice appears to remain relatively constant throughout the period tested, yet IR^lox/lox mice show an increase in threshold at 13 weeks. No significant difference between IR^lox/lox and SNIRKO mice was observed in beamwalk (C) or rotorod (D). Mechanical sensitivity, thermal sensitivity, and beamwalk were analyzed with a repeated measures 2-way ANOVA and Bonferroni’s post-hoc. Rotorod was analyzed with a student’s t-test. n=24 IR^lox/lox and n=23 SNIRKO. *=p<0.05.