Table 1.
The effect of flgD and flgE alleles on flagellar class 3 (fliC–lac) transcription in the presence and absence of the LP-rings and RflH/Flk.
| Strain set | Rod/hook mutation | Relative β-galactosidase activity
|
|||
|---|---|---|---|---|---|
|
rflH/flk+
|
rflH/flk−
|
||||
| flgH+I+ | ΔflgHI | flgH+I+ | ΔflgHI | ||
| 1. | None | 100 | 2 | 100 | 43 |
| 2. | flgD(S+A−) | 4 | 2 | 18 | 9 |
| 3. | ΔflgD | 3 | 2 | 21 | 11 |
| 4. | flgE(S+A−) | 3 | 1 | 9 | 11 |
| 5. | ΔflgE | 4 | 2 | 9 | 18 |
| 6. | ΔflgDE | 4 | 2 | 26 | 15 |
| 7. | ΔflgJ | 3 | 2 | 11 | 5 |
| 8. | ΔfliKa | 79 | 2 | 74 | 18 |
a
This ΔfliK allele is the in-frame deletion of amino acids 129 through 159 from strain MY2807 (Williams et al., 1996).
The S+A− designations for strains 2 and 4 represent secretion-proficient, assembly-deficient mutants in flgD and flgE respectively. The β-galactosidase activities were assayed twice in triplicate. The β-galactosidase activities of the wild-type strains were set to 100 and the average for the two independent sets of triplicate assays were normalized to the wild-type as described (Maloy, 1990).