Table 2.
Strains and plasmids used in this study.
| Strains and plasmids | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli | ||
| BL21(DE3)pLysS | Overproduction of proteins | Novagen |
| Salmonella | ||
| SJW1103 | Wild type for motility and chemotaxis | Yamaguchi et al. (1984) |
| SJW1103RflH | ΔrflH∷tetRA | This study |
| SJW156 | flgD | Ohnishi et al. (1994) |
| SJW156RflH | flgD ΔrflH∷tetRA | This study |
| SJW2177 | flgK | Homma et al. (1984) |
| MMEK001 | ΔflgE ΔfliK | This study |
| MMEK001RflH | ΔfigE ΔfliK ΔrflH∷tetRA | This study |
| MY2807 | Δfl/K(129–159) | Williams et al. (1996) |
| NME001 | ΔflgE | This study |
| NME001RflH | ΔflgE ΔrflH∷tetRA | This study |
| TH8426 | ΔfliK6140(6–405) | Minamino et al. (2006) |
| TH8426RflH | ΔfliK6140 ΔrflH∷tetRA | Minamino et al. (2006) |
| TH14136 | ΔflgHI958 fliC5050∷MudJ | This study |
| TH14137 | ΔflgHI958 fliC5050∷MudJ flk-5212∷Tn 10dTc | This study |
| TH14140 | fliC5050∷MudJ | This study |
| TH14141 | ΔflgHI958 ΔflgJ7677 fliC5050∷MudJ | This study |
| TH14142 | flgD157(S+A−) ΔflgHI958 fliC5050∷MudJ | This study |
| TH14144 | ΔflgD6541 ΔflgHI958 fliC5050∷MudJ | This study |
| TH14145 | flgE1353(S+A−) ΔflgHI958 fliC5050∷MudJ | This study |
| TH14146 | ΔflgE7616 ΔflgHI958 fliC5050∷MudJ | This study |
| TH14147 | ΔflgDE7639 ΔflgHI958 fliC5050∷MudJ | This study |
| TH14148 | flk-5212∷Tn 10dTc fliC5050∷MudJ | This study |
| TH14149 | ΔflgHI958 ΔflgJ7677 flk-5212∷Tn 10dTc fliC5050∷MudJ | This study |
| TH14150 | flgD157(S+A−) ΔflgHI958 flk-5212∷Tn 10dTc fliC5050∷MudJ | This study |
| TH14152 | ΔflgD6541 ΔflgHI958 flk-5212∷Tn10dTcfliC5050∷MudJ | This study |
| TH14153 | flgE1353(S+A−) ΔflgHI958 flk-5212∷Tn10dTcfliC5050∷MudJ | This study |
| TH14154 | ΔflgE7616 ΔflgHI958 flk-5212∷Tn10dTcfliC5050∷MudJ | This study |
| TH14155 | ΔflgDE7639 ΔflgHI958 flk-5212∷Tn10dTcfliC5050∷MudJ | This study |
| TH14211 | flgD157(S+A−) fliC5050∷MudJ | This study |
| TH14212 | ΔflgD6541 fliC5050∷MudJ | This study |
| TH14213 | flgE1353(S+A−) fliC5050∷MudJ | This study |
| TH14214 | ΔflgE7616 fliC5050∷MudJ | This study |
| TH14215 | ΔflgDE7639 fliC5050∷MudJ | This study |
| TH14216 | ΔflgJ7598∷tetRΔ fliC5050∷MudJ | This study |
| TH14218 | flgD157(S+A−) flk-5206∷Tn10dCm fliC5050∷MudJ | This study |
| TH14219 | ΔflgD6541 flk-5206∷Tn10dCm fliC5050∷MudJ | This study |
| TH14220 | flgE1353(S+A−) flk-5206∷Tn 10dCm fliC5050∷MudJ | This study |
| TH14221 | ΔflgE7616 flk-5206∷Tn10dCm fliC5050∷MudJ | This study |
| TH14221 | ΔflgDE7639 flk-5206∷Tn10dCm fliC5050∷MudJ | This study |
| TH14223 | ΔflgJ7598∷tetRΔ flk-5206∷Tn 10dCm fliC5050∷MudJ | This study |
| TH16207 | ΔfliK(129-159) fliC5050∷MudJ | This study |
| TH16208 | ΔflgHI958 Δfl/K(129-159) fliC5050∷MudJ | This study |
| TH16209 | ΔfliK(129-159) flk-5212∷Tn 10dTc fliC5050∷MudJ | This study |
| TH16210 | ΔflgHI958 ΔfliK(129-159) flk-5212∷Tn 10dTc fliC5050∷MudJ | This study |
| Plasmids | ||
| pET19b | Expression vector | Novagen |
| pGEX-6p-1 | Expression vector, GST | GE Healthcare |
| pBGIKN | pET19b/His-FliK(1-147) | Minamino et al. (2004) |
| pBGIKC | pET19b/His-FliK(265-405) | Minamino et al. (2004) |
| pKM002 | pTrc99Δ/FliK | Muramoto et al. (1998) |
| pKM003 | pET19b/His-FliK | Minamino et al. (1999) |
| pMM711 | pET19b/His-FlgD | Minamino and Macnab (2000a) |
| pMM1601 | pET19b/His-FlgE | Minamino and Macnab (2000a) |
| pMMIK2807 | pTrc99Δ/FliKΔ(129-159) | Minamino et al. (1999) |
| pMMIK2807ET | pET19b/His-FliKΔ(129-159) | This study |
| pNM001 | pTrc99Δ/FlgE | Moriya et al. (2006) |
| pNM201 | pTrc99Δ/FliKΔ(1-99) | This study |
| pRcD001 | pTrc99Δ/FlgD | Hirano et al. (2003) |