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. Author manuscript; available in PMC: 2019 Feb 20.
Published in final edited form as: Free Radic Biol Med. 2017 Dec 29;116:1–10. doi: 10.1016/j.freeradbiomed.2017.12.034

Figure 2.

Figure 2

Flow cytometry analysis for oxidative stress. (A–B) Forward scatter histograms. (C–D) 4-HNE staining histograms. H520 cells (A and C) and A431 cells (B and D) treated with 4-HNE antibody labeled dye were examined by flow cytometry (black line: untreated, red line: NIR PIT-treated). A and B indicate expanded cell size only in NIR PIT-treated A431 cells. C and D indicate that oxidative stress marker 4-HNE is stained only in NIR PIT-treated A431 cells.