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. 2018 May 22;13(5):e0197566. doi: 10.1371/journal.pone.0197566

Fig 7. Effects of human VEGF165 and HGF on IL-8 and MCP-1 production by endothelial cells in vitro.

Fig 7

A—MCP-1 production by HUVEC after stimulation with VEGF165, HGF or both factors over 4–12 hrs. Student’s t-test (Mean±S.D.; n = 4): * increased MCP-1 compared vs. HSA (p<0.05); # decreased MCP-1 compared vs. HSA or VEGF (p<0.01); ## decreased MCP-1 compared vs. VEGF (p<0.03). B—Autoradiograms of membrane stained for pIκBα и IκBα after Western blotting of extracts from HUVEC stimulated by VEGF165 (25 ng/ml), HGF (25 ng/ml) or VEGF165+HGF (25 ng/ml total); HSA (25 ng/ml) served as a negative, and TNF-α (5 ng/ml) as a positive control. C—IL-8 production by HUVEC after stimulation with VEGF165, HGF or both factors over 4–12 hrs. Student’s t-test (Mean±S.D.; n = 3–4): * increased IL-8 compared vs. HSA (p<0.05); ** increased IL-8 compared vs. VEGF or HGF (p<0.001); *** increased IL-8 compared vs. VEGF or HGF (p<0.01). D—Activation of HIF and IL-8 promoter in EA.hy926 after treatment with VEGF, HGF and their combination. Data of luciferase-based reporter assay (see Materials and methods for detailed description) Mann-Whitney U-test (Mean±S.D.; n = 3–4): *p<0.05 vs HSA; **p<0.025 vs. VEGF or HGF.