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. 2018 May 22;13(5):e0197846. doi: 10.1371/journal.pone.0197846

Fig 2. Respiratory rate (RR) per minute of adult zebrafish when treated with different anaesthetic protocols.

Fig 2

A) RR after loss of equilibrium and B) RR after loss of soft stimulus response of adult zebrafish after being subjected to an anaesthetic bath of: 100 μg/mL MS-222 (MS), 0.2 μg/mL etomidate (E); 0.2 μg/mL etomidate + 100 μg/mL lidocaine (E+L); 1.25 μg/mL propofol (P); 1.25 μg/mL propofol + 100 μg/mL lidocaine (P+L); 100 μg/mL ketamine (K); 100 μg/mL ketamine + 1.25 μg/mL medetomidine (K+M); or 100 μg/mL ketamine + 1.25 μg/mL medetomidine / 3.125 μg/mL atipamezole (K+M/A). Each point represents an animal (n = 8). Data are expressed as median [interquartile range]. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 when compared with MS-222. Non-parametric Kruskal-Wallis test followed by Dunn's multiple comparisons test.