Fig 3.
The pH based activity curve for WbDHFR (A.) and BmDHFR (B.). Enzyme concentrations in the wells were 11 nM WbDHFR and 37 nM BmDHFR. The wells also contained 100 μM NADPH, 100 μM DHF in 1 X MTEN buffer with pH values ranging from 5.5 to 9.0.
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