Table 1. Co-purified host proteins with p33 from yeast expressing Ufe1ΔTM.
| Protein | Pro-viral function | percentage |
|---|---|---|
| Ded1 helicase | (+)-strand synthesis | 98±10 |
| Cdc34 E2 enzyme, ub | Ubiquitination of p33 | 109±14 |
| Osh6 ORP (OSBP1-like) | oxysterol binding | 59±24 |
| Pbp2 RNA binding | Not known | 122±15 |
| Rpn11 deubiquitinase | Recruitment of Ded1 | 103±6 |
| Pex19 | Targeting of p33 to peroxisomes | 56±13 |
| RH2 DEAD-box helicase | (+)-strand synthesis | 70±18 |
| Tdh2 GAPDH | (+)-strand synthesis | 64±17 |
| Tef1 eEF1A | VRC assembly, (-)-strand synthesis, p33 stability | 67±25 |
| Vap27-1 (Scs2-like) | MCS formation, sterol | 68±30 |
| Vps4 AAA ATPase | VRC assembly | 44±27 |
| Vps23 ESCRT I | VRC assembly | 106±7 |
The amount of a given host protein co-purified with p33 from WT yeast (lacking Ufe1ΔTM) is taken as 100%. Each purification was repeated at least 3 times.