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. 2018 Apr 23;131(8):jcs210476. doi: 10.1242/jcs.210476

Fig. 1.

Fig. 1.

Substratum stiffness and Wnt3a synergistically regulate YAP/TAZ nuclear localization and activity. (A) Phase-contrast images of NMuMG cells cultured on soft (E≈130 Pa) or stiff (E≈4020 Pa) fibronectin-coated polyacrylamide substrata. Scale bars: 50 μm. (B) Fluorescence images of cells stained for YAP/TAZ (red) and nuclei (blue). Scale bars: 10 μm. (C) Quantification of the nuclear-to-cytoplasmic ratio of YAP/TAZ (n=3; >60 cells per experiment). The box represents the 25–75th percentiles, and the median is indicated. The whiskers show the 5–95th percentiles. (D–G) Relative transcript levels of (D) Ctgf, (E) Birc5, (F) Ankrd1 and (G) Cyr61. Error bars represent s.e.m. *P<0.05; **P<0.01; ***P<0.001; ns, not significant.