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. 2018 Apr 13;131(8):jcs211573. doi: 10.1242/jcs.211573

Fig. 5.

Fig. 5.

SHRC knockdowns exhibit premature ooplasmic streaming and actin defects. (A-D′) Single time-point and 30 time-point projections of live time-lapse movies of stage 7 oocytes in CCDC53RNAi(1) (A,A′), StrumpRNAi(1) (B,B′), SWIPRNAI(1) (C,C′), FAM21RNAi(1) (D,D′). (E) Quantification of the percentage of stage 7 SHRC RNAi oocytes exhibiting premature ooplasmic streaming. (F-I) Posterior cortex of stage 7 egg chambers stained for F-actin (Phalloidin) in CCDC53RNAi(1) (F), StrumpRNAi(1) (G), SWIPRNAI(1) (H), FAM21RNAi(1) (I). Note aberrantly long actin projections from cortex into oocyte (red arrows). (J-M) Cross sections of ring canals bridging the oocyte and a nurse cell in stage 7 egg chambers, stained for F-actin (Phalloidin) in CCDC53RNAi(1) (J), StrumpRNAi(1) (K), SWIPRNAI(1) (L), FAM21RNAi(1) (M). Red arrows denote inner actin ring canal and red arrowheads denote outer actin ring. Note disrupted actin structures surrounding intact ring canals (yellow arrow in K). (N-Q) Oblique view of ring canals bridging two nurse cells in stage 9 egg chambers, stained for F-actin (Phalloidin), in CCDC53RNAi(1) (N), StrumpRNAi(1) (O), SWIPRNAI(1) (P), FAM21RNAi(1) (Q). Note disorganized outer actin rings (brackets). (R) Quantification of the percentage of egg chambers exhibiting actin defects. Two-tailed Fisher's exact test (E,R). Sample size (N) indicated in bar plots in E and R. Scale bars: 50 µm in A-D′; 25 µm in F-I; 5 µm in J-Q.