Fig. 5.

In model flies fed with daunorubicin, Mbl transcript levels were not modified but Mbl-dependent splicing defects were restored. (A) RT-qPCR to assess Mbl expression levels relative to endogenous tubulin in cardiomyocytes showed that it was not modified by administration of daunorubicin. (B) RT-qPCR results of Serca exon 13 expression relative to Rp49 confirmed a significant rescue of the expression of this exon in model flies fed with daunorubicin. (C) Semi-quantitative PCR to assess inclusion of Fhos exon 16′ in model flies fed with DMSO or daunorubicin. Rp49 transcripts were detected as endogenous control. Quantification of percentage of exon inclusion (see D for a representative result) confirmed an improvement of Fhos mis-splicing in model flies fed with daunorubicin. *P<0.05, ***P<0.001 (Student's t-test).