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. Author manuscript; available in PMC: 2018 May 22.
Published in final edited form as: Biochemistry. 2018 Mar 23;57(14):2121–2131. doi: 10.1021/acs.biochem.7b01306

Table 3.

Thermodynamic Parameters for Hairpins in 1 M NaCl.a

Experiment Loop Formationb
sequence TM a ΔG°37 ΔH° ΔS° ΔG°37 ΔH° ΔS°
(°C) kcal/mol kcal/mol eu kcal/mol kcal/mol eu
5′GGCGAAUGCC 64.9 –2.47 –29.86 –88.31 4.21 –1.59 –18.71
5′CGGGCUUCCG 49.6 –0.86 –21.99 –68.14 4.76 2.04 –8.74
5′GGACGCUUGUCCa 70.9 –4.478 –45.078 –130.878 3.4 –7.77 –36.1
5′GGACUUCGGUCCa 76.4 –6.378 –55.978 –159.978 1.6 –18.67 –65.2
GGCUUCGGCC 72.2 –4.179 –40.179 –116.179 2.6 –11.8 –46.5
a

TM is calculated from ΔH° and ΔS°.

b

Loop parameters for Antao and Tinoco78 were recalculated with Watson-Crick nearest neighbor parameters of Xia et al.70