Figure 5.
A) Heat treatment (56 × 30 mins) in depolarizing protein- A-eluates from diabetic nephropathy/nonischemic cardiomyopathy or depression caused gain in N2A neurite outgrowth inhibition;
B) elution profile of neurite-inhibitory activity in the heat-treated diabetic nephropathy/nonischemic cardiomyopathy protein- A-eluates following gel filtration on Sephadex G75 as described in Methods. A) Data are mean + SE; * P < 0.05 comparing activity in depolarizing eluates before and after heating; or comparing (depolarizing vs non-depolarizing) eluates before; or after heating. B) Similar results were obtained following gel filtration of the heat-treated Pt 11 protein-A-eluate or unheated protein-A-eluate from a diabetic schizophrenia patient with nephropathy and dementia.