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. 2018 May 1;7:e36258. doi: 10.7554/eLife.36258

Figure 3. LC8-dLBD interactions monitored by ITC.

Figure 3.

(a) Construct schematics of the dLBD, QT1-3, QT2-4, QT4-6, and QT4-7 are shown, along with the locations of each TQT motif. A representative isothermal titration calorimetry thermogram of LC8 with the dLBD is shown on the right. (b) Representative thermograms of LC8 with constructs corresponding to QT1-3, QT2-4, QT4-6, and QT4-7. (c) Representative isothermal titration plots of LC8 with peptides corresponding to QT1p, QT2p, QT3p, QT4p, QT5p, QT6p, and QT7p. Since the interaction with QT3p is too weak, the heat exchange with buffer is plotted on top to show the difference between QT3p with LC8 and buffer. Higher concentrations were not possible due to poor solubility of the QT3p. Data were fit to a single site binding model using Origin software.