Table 2. Thermodynamic parameters of peptide-LC8 interactions.
| Peptide | Peptide Sequence*,† | N | Kd (μM) | ΔH (kcal/mol) | TΔS (kcal/mol) | ΔG(kcal/mol) |
|---|---|---|---|---|---|---|
| QT1p | ymssQKLDMETQTEe | 1.1 | 14 ± 3.5 | −5.9 ± 0.3 | 0.7 ± 0.4 | −6.6 ± 0.3 |
| QT2p | ylapLLRDIETQTPd | 1.0 | 7 ± 0.4 | −9.2 ± 0.5 | −2.2 ± 0.6 | −7.0 ± 0.4 |
| QT3p | ytpdTRGDIGTMTDd | --- | weak | ----- | ----- | ----- |
| QT4p | dlqTSAHMYTQTCd | 1.1 | 15 ± 0.8 | −8.7 ± 0.4 | −2.1 ± 0.5 | −6.6 ± 0.3 |
| QT5p | eelGLSHIQTQTHw | 0.9 | 11 ± 0.6 | −8.8 ± 0.4 | −2.0 ± 0.5 | −6.8 ± 0.3 |
| QT6p | wpdgLYNTQHTQTCd | 1.1 | 20 ± 1.0 | −8.6 ± 0.4 | −2.2 ± 0.5 | −6.4 ± 0.3 |
| QT7p | epdNFQSTCTQTRw | 1.1 | 10 ± 0.5 | −7.8 ± 0.4 | −0.9 ± 0.5 | −6.9 ± 0.3 |
*the 10-amino acid LC8 binding motif is capitalized.
†non-native residues added to the N-terminus of each peptide to increase solubility or improve concentration determination are underlined.