Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (human) | CAMK2A Calcium/Calmodulin Dependent Protein Kinase II Alpha | Uniprot: Isoform B (identifier: Q9UQM7-2) | Q9UQM7-2 | |
| Gene (C. elegans) | unc-43 Calcium/Calmodulin-Dependent Protein Kinase type II | Protein UNC-43 isoform d (Wormbase CDS K11E8.1d) | K11E8.1d | |
| Strain, strain background (C. elegans) | Worm (C. elegans) N2 Bristol Strain;unc-43(e408) | Caenorhabditis Genetics Center (CGC) PMID: 17941711 | 17941711 | |
| Recombinant DNA | pCDH-CMV-MCS-EF1α-Neo | Systems Biosciences (SBI) | CD514B-1 | |
| Recombinant DNA | pSM vector (a derivative of pPD49.26 with additional cloning sites) | Modified for this paper | NA | ADDGENE https://media.addgene.org/cms/files/Vec95.pdf |
| Recombinant DNA | pCDH-CMV-CAMK2A-T2A-mCherry | This paper; based on pCDH-CMV-MCS-EF1α-Neo | NA | |
| Recombinant DNA | pMIG-hOCT4 | Addgene | Plasmid #17225 | |
| Recombinant DNA | MSCV h c-MYC IRES GFP | Addgene | Plasmid #18119 | |
| Recombinant DNA | pMIG-hKLF4 | Addgene | Plasmid #17227 | |
| Recombinant DNA | pMIG-hSOX2 | Addgene | Plasmid #17226 | |
| Cell line (human) | Patient derived iPS neurons | This paper | NA | |
| Cell line (human) | 293T | Lab stock | NA | |
| Chemical compound, drug | MG132 | Sigma-Aldrich | M7449 | |
| Commercial kit | NativeMark PAGE | ThermoFisher | BN1002BOX | |
| Commercial kit | TnT Quick Coupled Transcription/Translation System | Promega | L1170 | |
| Antibody | Anti-FLAG Clone M2 | Sigma-Aldrich | F3165 | |
| Antibody | Anti-HA Clone Y-11 | Santa Cruz Biotechnology | sc-7392 | |
| Antibody | Anti-GADPH | Santa Cruz Biotechnology | sc-47724 | |
| Antibody | Anti-Tuj1 | Covance Research | MMS-435P | |
| Antibody | Anti-MAP2 | Synaptic Systems | 188 004 | |
| Cell culture reagent | 20% Knock Out Serum Replacement | Thermo Fisher | 10828–028 | |
| Cell culture reagent | bFGF | Stemgent | 37316 | |
| Cell culture reagent | Matrigel Basement Membrane Matrix | Corning | 354234 | |
| Cell culture reagent | mTeSR1 | STEMCELL Technologies | 85850 | |
| Cell culture reagent | CytoTune-iPS 2.0 Sendai Reprogramming Kit | ThermoFisher | A16517 | |
| Cell line (human) | H1 embryonic stem cells | Gift from Dr. Lawrence W. Stanton, WiCell | RRID:CVCL_C813 | |
| Antibody | Alexa Fluor 594 secondary Ab | ThermoFisher | Cat# A-11076, RRID:AB_2534120 | |
| Antibody | Alexa Fluor 488 secondary Ab | ThermoFisher | Cat# A-11001, RRID:AB_2534069 | |
| Assay system/kit | Maestro MEA System | Axion Biosystem | - |