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. 2018 May 22;7:e36491. doi: 10.7554/eLife.36491

Figure 7. Conditions that induced T. gondii tissue cyst formation in vitro did not induce tissue cyst formation in H. hammondi.

Figure 7.

(A) Representative images of cells infected with T. gondii (TgVEG) and H. hammondi (HhCatAmer) sporozoites that were grown for 2 days in pH 7.2 media and switched to pH 8.2 media (bradyzoite induction conditions) for 2 days, fixed and stained with an Anti-Toxoplasma gondii/Hammondia hammondi antibody and DBA. Scale bar represents 5 µm. (B) Quantification of T. gondii and H. hammondi tissue cyst formation described in A demonstrating that H. hammondi does not form tissue cysts when exposed to conditions that promote tissue cyst formation in T. gondii. Statistical significance was determined using a 2-way ANOVA with Tukey’s multiple comparison test. (****=P < 0.0001). (C) Number of parasites per vacuole for both T. gondii and H. hammondi grown at pH 7.2 and pH 8.2. T. gondii has significantly more parasites per vacuole than H. hammondi at both pH 7.2 and pH 8.2. There is no statistically significant difference between either T. gondii or H. hammondi between pH 7.2 and pH 8.2. Statistical significance was determined using a 2-way ANOVA with Sidak’s multiple comparison test. (**=P < 0.01 and ****=P < 0.0001).