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. 2018 May 22;7:e36491. doi: 10.7554/eLife.36491

Figure 8. The H. hammondi transcriptome is refractory to pH-induced stress.

(A) GSEA analysis comparing TgVEG (blue) and HhCatAmer (red) treated with control media to TgVEG and HhCatAmer treated with pH 8.2 media. Not all gene sets are significantly enriched. NES: Normalized enrichment score FDR q: False discovery rate q-value. See table supplement for FDR q-values for all analyses. Nominal p-value is indicated by *<0.05, **<0.01, and ***<0.001. (B) GSEA plots of the IN VITRO BRADYZOITE gene set comparing control versus pH 8.2 treated TgVEG and HhCatAmer. (C) Heat map showing mean centered log2(FPM) values for all detectable genes from the IN VITRO BRADYZOITE gene set. (D) Mean centered, hierarchically clustered heatmap of log2(FPM) values of the 24 genes that significantly differed in transcript abundance (fold-change ≥1; Padj <0.01) between control and pH 8.2 treated H. hammondi. As shown only three transcripts were found to be of higher abundance in pH-treated H. hammondi, while the remaining genes were of significantly lower abundance after pH exposure.

Figure 8.

Figure 8—figure supplement 1. Transcriptional profiling and qPCR of pH vs control RNAseq data.

Figure 8—figure supplement 1.

(A) Summary of reads mapping from each sample for T. gondii and H. hammondi zoites treated with control or bradyzoite-induction media. (B) Fold change in transcript abundance relative to control of canonical bradyzoite genes ENO1, BAG1, and LDH2 determined by qPCR for TgVEG and HhCatAmer exposed to pH 8.2. *=P < 0.05 and **=P < 0.01.