(A) Averaged p-Mad intensity profiles of wild-type tkvmCherry (n = 20), tkvC40Y-mCherry/+ (n = 24), tkvC90R-mCherry/+ (n = 24), tkvC97-mCherry/+ (n = 24), and tkvM442T-mCherry/+ (n = 16) wing discs. (B) Wing disc size comparisons. wild-type tkvmCherry (nhet = 44; nhomo = 31), tkvC40Y-mCherry (nhet = 52; nhomo = 32), tkvC90R-mCherry (nhet = 35; nhomo = 32), tkvC97R-mCherry (nhet = 42; nhomo = 23), tkvR409C-mCherry (nhet = 21; nhomo = 31), and tkvM442T-mCherry (nhet = 37; nhomo = 33). (C–H) tkvR409C homozygous animals developed normal wings. Wing discs and adult wings from wild-type tkvmCherry (C–E) and tkvR409C-mCherry (F–H) homozygous animals. Scale bars: 50 μm for (C), 0.5 mm for (E). Anterior is oriented to the left in (C), (D), (F and G), and to the top in (E and H).