. Author manuscript; available in PMC: 2019 May 1.

Published in final edited form as: Biochim Biophys Acta. 2018 Jan 3;1860(5):1069–1076. doi: 10.1016/j.bbamem.2018.01.001

Table 3.

Partitioning constant (K_d) values for various mutants involving POPC vesicles containing or lacking MonoGlycCer. Partitioning constants (K_d) were determined from the fluorescence intensity changes as described in the Exptl. Proc.

	K_d (μM)
	GalCer	GlcCer	POPC
wtHET-C2	0.11 ±0.1^*	0.13 ±0.1^*	5.26 ±1.39^*
W208A	0.21 ±0.03	0.74 ±0.08	3.05 ±0.81
W208F	0.10 ±0.02	0.88 ±0.07	3.05 ±0.46
F149A	4.97 ±0.40	2.63 ±0.13	3.57 ±1.04
F149Y	3.79 ± 1.01	1.95 ±0.28	9.43 ±4.50

Values for wtHET-C2 determined previously by Kenoth et al. [8] were comparable to newly calculated values shown in Figure S2.