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. Author manuscript; available in PMC: 2018 Oct 23.
Published in final edited form as: Nat Chem Biol. 2018 Apr 23;14(6):591–600. doi: 10.1038/s41589-018-0044-1

Figure 1. Characterization of the engineered monomeric miRFP720.

Figure 1

(a) Fluorescence excitation and (b) fluorescence emission spectra of miRFP720 (magenta) overlaid with spectra of other monomeric NIR FPs, such as miRFP670 (blue), miRFP703 (green) and miRFP709 (red). (c) NIR fluorescence brightness of live HeLa cells transiently transfected with miRFP720 (magenta, open bar) compared to that of parental dimeric iRFP720 (magenta, shaded bar) and other monomeric NIR FPs, such as miRFP670 (blue), miRFP703 (green), miRFP709 (red) and mIFP (orange), analyzed using flow cytometry. NIR fluorescence intensity was normalized to transfection efficiency (fluorescence intensity of co-transfected EGFP), to excitation efficiency of each NIR FP with 635 nm laser, and to fluorescence signal of each NIR FP in the emission filter. Error bars, S.D. (mean of n=3; independent experiments).