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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Arch Toxicol. 2018 Apr 6;92(5):1767–1783. doi: 10.1007/s00204-018-2185-0

Figure 7. Incorporation of unsaturated fatty acids in the lipid raft entities following enrichment of A549-cells with PUFA/DHA.

Figure 7

(A) (i) Cell viability on enrichment with PUFA/DHA complexed in (Fatty acid free) BSA in cultures maintained in media with or without FBS (n=3-4/group).(ii) Dot blots of lipid raft fractions from PUFA/DHA-enriched or control A549 cells used for lipidomic analysis.(B) Levels of (i) DHA, or (ii) unsaturated fatty acids detected in lipid raft fractions isolated from PUFA/DHA-enriched or control A549 cells on lipidomic analysis. Equal amount of protein were used for lipid raft purification to normalize the results. (n=3-4/group). Results are a representation of three independent experiments. Error bars represent SEM, * (p<0.05), as per two tailed student t-test.