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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Dev Biol. 2018 Apr 19;439(2):102–111. doi: 10.1016/j.ydbio.2018.04.012

Figure 1. Foxd1 is broadly expressed in hypothalamic and prethalamic neuroepithelial cells.

Figure 1

A–D: Schematic showing Foxd1 mRNA expression in the anterior hypothalamus and prethalamus over the course of development, adapted from Shimogori et al., 2010. E: GFP staining from a Foxd1-Cre/+; Ai9 E8.5 embryo. White arrowheads indicate the presumptive hypothalamus in the ventral neural tube. F, G: Whole mount in situ hybridization of Foxd1 mRNA at E9.0 (F) and E10.0 (G). The hypothalamus is designated by white arrowheads. H: Immunohistochemistry staining against GFP in a Foxd1-Cre/+ E12.5 brain detects expression of Cre-GFP fusion protein expressed from the Foxd1 locus. By this age, Foxd1 is expressed only in the prethalamus (open arrowhead) and the anterior hypothalamus (white arrowhead) along the ventricle. I–K. Despite the limited Foxd1 expression at E12.5, lineage tracing in E12.5 Foxd1-Cre/+; Ai9 brains reveals that every cell in the hypothalamus and prethalamus originates from a Foxd1-positive lineage. Open arrowheads=prethalamus, white arrowheads=hypothalamus. L–S: DsRed immunohistochemistry against in P0.5 Foxd1-Cre/+; Ai9 brain reveals a history of Cre activity throughout the prethalamus and hypothalamus. Blue=DAPI. Open arrowheads=prethalamus, white arrowheads=SCN. Sections are arranged in anterior-posterior sequence from top left to bottom right.