Table 2. RNA-Seq analysis of control and Foxd1-deficient E17.5 hypothalamus.
RNA-Seq analysis of E17.5 hypothalamus from heterozygote control and Foxd1 mutants. Genes are listed that show significant differential expression and/or were analyzed by in situ hybridization. Differentially expressed genes were determined by q-value<0.05. Transcription factors and coregulators are highlighted in italic.
| Gene | Fold Change (log2) | q-value | Expression | In situ validated? |
|---|---|---|---|---|
| Avp | −3.38 | 0.04 | PeVN, SCN, PVN | yes |
| Foxd1 | −3.22 | 0.04 | TARGET | no |
| Oxt | −2.49 | 0.04 | PeVN | no |
| Slc6a3 | −1.65 | 0.04 | ZI/AH | no |
| 4930555G | ||||
| 01Rik | −1.49 | 0.04 | High in AH | no |
| Vipr2 | −1.32 | 0.49 | SCN | yes |
| Crh | −1.29 | 0.04 | PeVN | no |
| Vax1 | −1.21 | 0.04 | AH | yes |
| Cdh23 | −1.04 | 0.04 | High in AH | no |
| Rora | −1.01 | 1 | SCN | yes |
| Rorb | −0.20 | 1 | SCN | yes |
| Th | −0.17 | 1 | SCN adjacent | yes |
| Six3 | −0.16 | 1 | AH | yes |
| Isl1 | −0.10 | 1 | AH | yes |
| Lhx1 | −0.10 | 1 | SCN | yes |
| Gad1 | 0.01 | 1 | AH | yes |
| Sst | 0.11 | 1 | PeVN | yes |
| Trh | 0.23 | 1 | AH | yes |
| Emx2 | 0.31 | 1 | SCN and PeVN | yes |
| Zbtb18 | 0.66 | 0.04 | vAH | no |
| Tbr1 | 0.77 | 0.04 | Telencephalon | no |
| Satb2 | 0.89 | 0.04 | Telencephalon | no |
| Eomes | 1.06 | 0.04 | EmThal | no |
| Foxg1 | 1.06 | 0.04 | Telencephalon | yes |
| Kdm5d | 1.17 | 0.04 | ND | no |
| Neurod2 | 1.18 | 0.04 | Telencephalon | no |