Fig. 2.

Insulin receptor (IR) and signaling pathways are activated in the kidney of old mice. a Kidney cortical lysates were employed in ELISA and showed decrease in phosphorylation of acetyl Co-A carboxylase (ACC) in old vs. young mice. Immunoblotting of renal cortical lysates showed the following in old vs. young mice: b, c, e–g increase in the phosphorylation of p70S6 kinase, eEF2 kinase (eEF2K), Akt, glycogen synthase kinase 3β (GSK-3β), and insulin receptor-β (IRβ) and d decrease in the phosphorylation of eukaryotic elongation factor 2 (eEF2). h, i ELISA showed increase in renal cortical tyrosine phosphorylation of IRβ and insulin receptor substrate-2 (IRS-2) in old vs. young mice. Data from young (n = 10) and old mice (n = 10) are shown as mean ± SEM in histograms