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. 2018 May 22;8:8018. doi: 10.1038/s41598-018-26333-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sequences of the amplicons obtained by NS2A JEV RT-qPCR from throat swabs of patients 5 and 10. Sequences obtained for the 2 patients were aligned (using ClustalX 2.1) with the corresponding sequences of all JEV strains ever handled in the laboratory. KF907505: JEV RNA used as positive control for all JEV RT-qPCR runs. KC196115 and CNS1326: the two JEV strains isolated on cell culture from CSF patients in 2009 and 2013 respectively. Sequence identities are indicated by stars. Sequences of the forward and reverse primers used for the JEV RT-qPCR are indicated above the sequence alignment.