Table 1.
Patients with positive results for JE MAC-ELISA or JEV RT-qPCR.
| Patient number | Age (yr) | Sex | JE MAC-ELISA (ISR)° | JEV RT-qPCR (Ct value) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Admission Serum | Convalescent Serum | CSF | Throat swab | CSF | Serum | ||||||
| NS2A | NS3 | NS2A | NS3 | NS2A | NS3 | ||||||
| 1 | 19 | M | Pos (18.4) | Pos (45.1) | Pos (29.8) | Neg | Neg | Neg | Neg | Neg | Neg |
| 2 | 50 | M | Pos (26.0) | Pos (40.8) | Pos (34.2) | Neg | Neg | Neg | Neg | Neg | Neg |
| 3 | 33 | M | Pos (40.6) | Neg (3.2) | Pos (35.1) | Neg | Neg | Neg | Neg | — | — |
| 4 | 7 | M | Pos (17.7) | — | Pos (44.4) | Neg | Neg | Neg | Neg | — | — |
| 5 | 16 | M | Pos (7.9) | Pos (18.0) | Pos (21.6) | Pos (32) | Pos (37) | Neg | Neg | — | — |
| 6 | 13 | M | Pos (11.3) | Pos (27.9) | Pos (60.3) | Neg | Neg | Neg | Neg | — | — |
| 7 | 14 | M | Pos (21.7) | — | Pos (38.2) | Neg | Neg | Neg | Neg | Neg | Neg |
| 8 | 3 | F | Pos (13.6) | — | Pos (33.9) | Neg | Neg | — | — | Neg | Neg |
| 9 | 3 | M | Pos (18.0) | — | Pos (41.8) | Neg | — | Neg | — | Neg | — |
| 10 | 13 | M | Pos (22.3) | — | Pos (42.8) | Pos (36) | — | Neg | — | Neg | — |
| 11 | 23 | M | Pos (6.3) | Neg (4.8) | Pos (6.4) | Neg | Neg | — | — | — | — |
M: male, F: female. CSF: cerebrospinal fluid. °Anti-JEV IgM detection using JE Detect™ IgM Antibody Capture ELISA Kit (InBios, Washington, USA). Positive (Pos) = Anti-JEV IgM positive, negative (Neg) = Anti-JEV IgM negative or equivocal, with ISR (Immune status Ratio) cut-offs calculated according to the manufacturer’s instructions. RT-qPCR negative (Neg) = no amplification curve or curve with a Cq > 40. RT-qPCR positive (Pos) = amplification curve with Cq < 40. “−“ = no sample available for testing due to small sample volume collected and/or the use in its entirety for previous assays.