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. 2018 May 22;8:8002. doi: 10.1038/s41598-018-26402-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RPA-LFS Detection of Diluted DNA. P1 represents the positive control for the Ginkgo biloba RPA-LFS detection system: detection result of the RPA amplification of DNA extracted from Ginkgo biloba leaves; N1 represents the negative control for the Ginkgo biloba RPA-LFS detection system. The following strips showed the amplification of Ginkgo biloba DNA diluted as indicated on the label. P2 represents the positive control for the Sophora japonica RPA-LFS detection system: detection result of the RPA amplification of DNA extracted from Sophora japonica leaves; N2 represents the negative control for the Sophora japonica RPA-LFS detection system. The following strips showed the results of amplifying Sophora japonica DNA diluted as indicated on the label. The concentrations of diluted DNA are presented under the strips.