Table 1.
Details regarding the primers and probes used in this study.
| Primers used for PCR amplification | ||||
|---|---|---|---|---|
| Name | Direction | Amplicon Size | Targeted Species | Sequence (5′-3′) |
| YXF | Forward | 114 | Ginkgo biloba | CGAGGAAGGTTCTGTTGCTA |
| YXR | Reverse | 114 | Ginkgo biloba | TTGGAATAAGCAGGAGGAAT |
| HMF | Forward | 101 | Sophora japonica | GAGTCTTTGAACGCAAGTTG |
| HMR | Reverse | 101 | Sophora japonica | GACGGCACGGATGCTTAA |
| Primers and probes used for RPA amplification | ||||
| YXSF | Forward | 176 | Ginkgo biloba | ATTCCTCCTGCTTATTCCAAAACTTGCCAGGGTCC |
| YXSR | Reverse | 176 | Ginkgo biloba | biotin-TCAAGTCCACCACGAAGACATTCGTAAACTGCT |
| YX-probe | Forward | / | Ginkgo biloba | FAM-AATATGGCCGTCCCCTATTGGGATGTACTATC-THF-AGCC AAAATTGGGTT-C3-spacer |
| HMSF | Forward | 132 | Sophora japonica | TAGGTCCTGAGCGGGGCGAATGTTGGCTTC |
| HMSR | Reverse | 132 | Sophora japonica | biotin-AGACACACGCGATTGGTCTCGAGATTTTACTCAGC |
| HM-probe | Forward | / | Sophora japonica | FAM-CTTCCCGTGAGCCTTGTCTCGCGGTTGGTT-dspacer-AAAAA TGTGTCTGTGG-C3-spacer |