Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 22;8:7984. doi: 10.1038/s41598-018-26095-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of CS exposure on CD4 Cell surface receptor quantified by single cell imaging flow cytometry. NHBE ALI cultures were exposed to CS. At the end of smoking, cells were immuno-stained with surface markers anti-Human CD4 labeled with FITC. % Gated CD4+ or FITC+ cells for air NHBE (22.71 ± 2.44%) and smoke NHBE (32.78 ± 2.71%) are represented (panel a). MFI of FITC from in total population of cell images acquired (10,000 per sample) for air NHBE cells (1597.65 ± 633.43) and smoked NHBE cells (3208.24 ± 1030.86) are represented (panel b). A representative overlay histogram of intensity of FITC for isotype control (yellow), air (blue) and smoke (green) (panel c). A representative single cell image for each sample (panel d). Experiments were carried out from at least 3 different lungs. *Significant (p < 0.05).