Fig. 7. Hirsutine inhibits tumor growth and induces apoptosis in A549 xenograft model.

Mice were subcutaneously inoculated with A549 cells (2 × 10⁶) into the right flanks and randomly divided into two groups (n = 10). Mice were injected intraperitoneally (i.p.) with 10 mg/kg/day hirsutine or an equal volume of vehicle. a The tumor growth curve of vehicle control mice and hirsutine-treated mice. **P < 0.01 vs. the control group. b Representative image of tumors of two groups. c There were no significant differences of body weights between vehicle control and hirsutine-treated groups. d Representative two tumor tissues from each group were fixed and subjected to hematoxylin and eosin (H&E) staining, and immunohistochemistry staining for C-CASP3 and p-GSK3β (Ser9). e Representative H&E-stained liver and kidney sections from two groups are shown. Scale bars: 25 μm. f Representative two tumor tissues from each group were prepared and subjected to western blot analysis