Fig. 2. Neuronal MCU deficiency reduces the loss of eYFP⁺ neurons in the anterior motor cortex.

Prominent eYFP⁺ labeling was detected in layer V of the anterior motor cortex with fewer eYFP⁺ neurons observed in layers II–IV and V a. The areas occupied by eYFP⁺ neurons were quantified in the region indicated by the blue box for the insert in Fig. 1J for TMX/SLICK-H and TMX/SLICK-H/MCU^fl/fl mice 24 h after sham HI or HI brain injury b. Relative to the representative TMX/SLICK-H/HI mouse c, eYFP⁺ neurons were preserved in the representative SLICK-H/MCU^fl/fl/HI mouse d. Adjacent sections show that the reductions in FJ⁺ cells were greatest in layer V of the anterior motor cortex of the TMX/SLICK-H/MCU^fl/fl/HI mouse e and f