Fig. 4. Immortalized DKO keratinocytes show a unique phenotype relative to GR^EKO, MR^EKO, and CO cells, with reduced cell size and altered proliferation.

a Representative phase contrast images of CO, DKO, GR^EKO, and MR^EKO keratinocytes. b Representative experiment for forward and side scatter evaluated by flow cytometry in CO, DKO, GR^EKO, and MR^EKO cells; n = 3. c Immunoblotting of cell lysates using E-cadherin, K5, p63, and tubulin antibodies. d. Growth kinetics of cultured CO, DKO, GR^EKO, and MR^EKO keratinocytes. Asterisks indicated statistically significant differences relative to CO: *p < 0.05; n > 4 biological replicates per genotype