Table 1.

Overview of MPS configuration, MPS-specific hardware and cell parameters.

MPS	Cardiac	Liver	Gut
Cell type	iPSC-derived cardiomyocytes	Human primary hepatocytes	Cancer cell line (differentiated Caco-2/HT-29)
Format	96-well	LiverChip	12-well transwell
Maturation/Differentiation duration post seeding (days)	2	3	21
Seeding density	0.3 × 106/cm2	6 × 105/scaffold	0.9 × 105/cm2 (9:1 ratio)
Operating media volume (ml)	0.2	2.1	Apical: 0.5 Basal: 1.5
Media volume per seeded cell (nl/cell)	2.1	3.5	17
Media flow	static	recirculation	static
Media change frequency	2 days	2 days	2 days