Fig. 1.

BAPTA and nocodazole inhibition do not affect LAT microcluster formation. a Jurkat cells were treated with DMSO and dropped onto stimulatory coverslips in a medium containing DMSO (control) or treated with BAPTA and dropped onto stimulatory coverslips in a medium containing EGTA (BAPTA), and fixed 2 and 5 min after dropping. Cells were then permeabilized and immunostained with pTyr and pLAT. Scale bar equals 2 μm. b Cluster area and c cluster intensity of pLAT microclusters were quantified. d Jurkat cells transfected with LAT-ruby were treated with DMSO and dropped onto stimulatory coverslips in a medium containing DMSO (control) or treated with nocodazole and dropped onto coverslips in a medium containing nocodazole (nocodazole) and fixed 2 and 5 min after dropping. Cells were then permeabilized and immunostained with pLAT. Scale bar equals 2 μm. e Cluster area and f cluster intensity of pLAT microclusters were quantified. See also Supplementary Figure 1